食品中の残留農薬分析におけるサンプル前処理時間を短縮させ、高い効率を実現する新製品（図１）

図 1 の拡大図

Agilent QuEChERS AOAC procedure		Agilent QuEChERS EN procedure
1.	Accurately weigh 15 g comminuted sample (±0.05 g) in 50 mL centrifuge tube	1.	Accurately weigh 10 g comminuted sample (±0.05 g) in 50 mL centrifuge tube
2.	Spike samples with 100 uL of IS solution, vortex for 1 min	2.	Spike samples with 100 uL of IS solution, vortex for 1 min
3.	Add 15 mL of 1% HAc in ACN, shake vigorously for 1 min	3.	Add 10 mL of ACN, shake vigorously for 1 min
4.	Add SampliQ QuEChERS AOAC salt packet, cap tubes shake vigorously for 1 min	4.	Add SampliQ QuEChERS EN salt packet, cap tubes shake vigorously for 1 min
5.	Centrifuge at 4000 rpm for 5 min	5.	Centrifuge at 4000 rpm for 5 min
6.	Transfer upper ACN layer to SampliQ AOAC dispersive SPE tube, 1 mL sample/2 mL tube or 8 mL sample/ 15 mL tube	6.	Transfer upper ACN layer to SampliQ AOAC dispersive SPE tube, 1 mL sample/2 mL tube or 6 mL sample/ 15 mL tube
7.	Vortex and centrifuge	7.	Vortex and centrifuge
8.	Transfer 200 uL extract to AS vial, dilute with 800 uL of appropriate solvent	8.	Transfer 200 uL extract to AS vial, add 10 uL of 1% FA in ACN, dilute with 800 uL of water
9.	Samples are ready for LC/MS/MS analysis	9.	Samples are ready for LC/MS/MS analysis
Abbreviations: IS = internal standard; HAc = acetic acid; ACN = acetonitrile; AS = autosampler; FA = formic acid

QuEChERS サンプル前処理手順により、サンプル前処理ステップが簡単になり、必要な溶媒の使用量やガラス器具が減少します。