The Agilent Seahorse XF Mito Tox Assay kit allows for the identification of drug-induced mitochondrial toxicity through direct functional measurements of mitochondrial oxygen consumption rates (OCR). This kit is adapted from the Agilent Seahorse XF Cell Mito Stress Test, the fundamental assay for interrogating mitochondrial function, with improved assay design and data outputs. It provides high sensitivity and specificity along with the toxicity mode of action in one assay, minimizing false positives and false negatives. The XF Mito Tox Assay Kit seamlessly integrates with Agilent Seahorse Pro Controller and Wave Pro software and Agilent Seahorse Analytics in the assay workflow, providing an end-to-end solution for assessing drug-induced mitochondrial toxicity. The software features greatly simplify the entire XF assay experience, from assay design or template creation to data interpretation, facilitating decision making to de-risk drug pipelines.
Each assay kit contains sufficient materials for six full-plate tests in 96-well format. The assay is only compatible with the Agilent Seahorse XF Pro analyzer.
High sensitivity and specificity for identification of mitochondrial toxicity through direct measurement of mitochondrial dysfunction
Information-rich data makes it possible to distinguish inhibitors and uncouplers in one assay
Improved assay robustness and throughput through the new features of the XF Pro analyzer, consumables, and assay design
Enhanced usability in assay template creation, data quality assessment, and data reporting process
Dedicated widgets in Seahorse Analytics that reports Mito Tox Indices (MTI), IC50/EC50, and Z automatically
Validated for use with Agilent Seahorse XF Pro analyzer and XF Pro M plate. Best performed with Agilent Seahorse XF DMEM medium, pH 7.4 (p/n 103575-100) and supplements (p/n 103577-100, 103578-100, 103579-100)
Compounds are reconstituted directly in the assay medium, eliminating the need for DMSO or other solvents
Every lot is rigorously tested and validated with functional assays to deliver consistent and robust results
Use this link to access Seahorse Analytics for data analysis: https://seahorseanalytics.agilent.com
This application note describes the use of human primary hepatocytes in the context of the Agilent Seahorse XF Mito Tox assay, including assay optimization,
This document provides instruction on how to perform FCCP optimization using the Agilent Seahorse XF Cell Mito Stress Test with the Agilent Seahorse XF Pro Analyzer
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