[ Themes ]
QC or Bust: Performance Assessment for Quantitative Plasma Proteomics by MRM
Christoph Borchers, Ph.D., University of Victoria – Genome BC Proteomics Centre, Victoria, BC, Canada
Defining the Next Experiment:
Pathway - directed Analysis from Protein Discovery Data
Christine Miller, Senior Application Scientist, Agilent Technologies
Learn how pathway knowledge can be used to create targeted proteomics experiments to verify and validate candidate disease biomarkers in complex matrices. Examples are shown in the seminar. For some targeted assays, absolute quantitation is key to finding the true biological differences. In order to enable the acquisition of more precise and accurate quantitative data, two reference kits were developed by MRM Proteomics using SIS peptide approach for undepleted and non-enriched human plasma.